Direct measurement of SR release flux by tracking ‘Ca2+spikes’ in rat cardiac myocytes
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چکیده
منابع مشابه
Frequency and release flux of calcium sparks in rat cardiac myocytes: a relation to RYR gating
Cytosolic calcium concentration in resting cardiac myocytes locally fluctuates as a result of spontaneous microscopic Ca(2+) releases or abruptly rises as a result of an external trigger. These processes, observed as calcium sparks, are fundamental for proper function of cardiac muscle. In this study, we analyze how the characteristics of spontaneous and triggered calcium sparks are related to ...
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In cardiac muscle, Ca(2+)-induced Ca(2+) release (CICR) from the sarcoplasmic reticulum (SR) defines the amplitude and time course of the Ca(2+) transient. The global elevation of the intracellular Ca(2+) concentration arises from the spatial and temporal summation of elementary Ca(2+) release events, Ca(2+) sparks. Ca(2+) sparks represent the concerted opening of a group of ryanodine receptors...
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ATP is released by numerous cell types in response to mechanical strain. It then acts as a paracrine or autocrine signaling molecule, inducing a variety of biological responses. In this work, we addressed the question whether mechanical force acting on the membranes of contracting cardiomyocytes during periodic longitudinal shortening can stimulate the release of ATP. Electrically stimulated is...
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In mammalian cardiac myocytes, calcium released into the dyadic space rapidly inactivates calcium current (ICa). We used this Ca2+ release-dependent inactivation (RDI) of ICa as a local probe of sarcoplasmic reticulum Ca2+ release activation. In whole cell patch-clamped rat ventricular myocytes, Ca2+ entry induced by short prepulses from -50 mV to positive voltages caused suppression of peak IC...
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ژورنال
عنوان ژورنال: The Journal of Physiology
سال: 1998
ISSN: 0022-3751
DOI: 10.1111/j.1469-7793.1998.677bd.x